Research Articles
CataPro Deep Learning: Revolutionizing Enzyme Kinetics Prediction for Drug Discovery and Metabolic Engineering
This article provides a comprehensive guide to CataPro, a cutting-edge deep learning model for predicting the critical enzyme kinetic parameters kcat and Km.
Mastering CRISPRi for Metabolic Pathway Regulation: A Comprehensive Guide for Research & Therapeutic Development
This article provides a comprehensive overview of CRISPR interference (CRISPRi) as a powerful tool for precise metabolic pathway regulation.
CRISPRi for Dynamic Metabolic Pathway Control: A Guide for Researchers in Biotechnology and Therapeutics
This article provides a comprehensive guide for researchers, scientists, and drug development professionals on implementing CRISPR interference (CRISPRi) for the dynamic, tunable, and reversible regulation of metabolic pathways.
Harnessing CRISPR/Cas9 in Aspergillus niger: A Comprehensive Guide to Heterologous Protein Expression for Industrial and Therapeutic Applications
This article provides a detailed roadmap for researchers and bioprocess engineers aiming to utilize CRISPR/Cas9 genome editing in the filamentous fungus Aspergillus niger for heterologous protein expression.
CATNIP Tool: Revolutionizing AKG-Dependent Enzyme Discovery for Drug Development
This article provides a comprehensive guide to the CATNIP (Conserved Active-site Typing for Natural Product discovery) computational tool, designed to predict and analyze alpha-ketoglutarate (AKG) and Fe(II)-dependent oxygenases and oxidases.
From Single Carbons to Complex Molecules: Engineering Multi-Enzyme Cascades for C1 to C2/C4 Biosynthesis
This article provides a comprehensive review of the design, application, and optimization of multi-enzyme cascades for converting single-carbon (C1) compounds like CO2, formate, and methanol into valuable C2 and C4...
The Brønsted-Evans-Polanyi Principle in Enzymology: From Theory to Drug Discovery Applications
This article explores the application and significance of the Brønsted-Evans-Polanyi (BEP) relationship in enzyme catalysis.
Accelerating Protein Engineering: A Practical Guide to Bayesian Optimization for Drug Discovery
This article provides a comprehensive guide to Bayesian Optimization (BO) for protein engineering, targeting researchers, scientists, and drug development professionals.
SABIO-RK vs. BRENDA Database Comparison: Choosing the Right Tool for Enzyme Kinetics Research
This comprehensive analysis compares the two primary public repositories for enzyme kinetics data, BRENDA and SABIO-RK.
Unlocking Enzyme Flexibility: A Comprehensive Guide to B-Factor Analysis for Dynamic Region Identification
This article provides researchers, scientists, and drug development professionals with a complete framework for using B-factor (temperature factor) analysis to identify flexible and dynamic regions in enzyme structures.